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As our control transfections resulted in viable colony formation in both A2780 and A2780DPP cells, we hypothesised that GSTP1 knockdown may be lethal in A2780DPP cells, which are grown in medium containing 1 μ M cisplatin to maintain the drug-resistant phenotype

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Nuclei were stained purple, and those with a minor axis length of more than 4 µm were counted as cells with round nuclei